JAK-STAT Control of Zika Virus-Induced Fetal Injury

$3.28M USD

Funder	NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
Recipient Organization	University of Washington
Country	United States
Start Date	Feb 10, 2021
End Date	Jun 30, 2021
Duration	140 days
Number of Grantees	2
Roles	Principal Investigator; Co-Investigator
Data Source	NIH (US)
Grant ID	`10321508`

Grant Description

Project Summary The current project, Jak STAT control of Zika virus-induced fetal injury (R01 AI143265), includes the processing of many nonhuman primate (NHP) and human tissue and cell samples for high throughput RNA sequencing (RNAseq) analysis.

Our sample-processing pipeline for these assays includes RNA isolation and quality control (QC), and library construction and library QC.

We currently utilize a Biomek FX automated workstation to perform the majority of these work steps in handling high volume samples in our high throughput workload for these analyses. The Biomek FX is 10-years old.

Due to the age, it is not reliable, the ability to obtain a future service contract is not guaranteed, and the current configuration and software are not compatible with off- the-shelf protocols. Thus, we are in need of funds to replace this instrument.

The purchase of a Biomek i7 Hybrid automated workstation to replace our Biomek FX workstation, allows us to achieve our stated project goals. 0