Single Cell RNA Sequencing (scRNA-seq) Instrumentation

PROJECT SUMMARY We propose to acquire a benchtop cell sorter which will be paired with a cell partitioner in order to establish a single cell RNA sequencing (scRNA-seq) sample preparation instrument This shared instrumentation provides maximum flexibility for investigators and will be the only instrument of its kind on the University of Arkansas (U

of A) campus and Northwest Arkansas area. The instrument will be housed within the Engineering Research Center, a facility with the infrastructure and personnel necessary to support its operation. In support of this effort, a cohort of 6 Major and 12 Minor Users have been identified that represent the largest individual needs

for single cell analysis capabilities at the U of A. These users represent a mix of established and early-career investigators and reflect the dynamic and growing need for single cell analysis. Together, the Major and Minor users hold grants from the NIH (10), NSF (4), USDA (2) and State sources (2) and are projected to comprise

approximately 85% of the instrument’s Accessible User Time (AUT) in the first year of operation. Additional usage is anticipated from additional pilot project users with less substantial (