Element Aviti- Mid throughput Short read sequencer for YCGA

Project Summary Yale Center for Genome Analysis (YCGA) has been providing cutting-edge next-generation sequencing services to support the fundamental research needs of hundreds of Yale and non-Yale NIH-funded investigators. YCGA has been offering short read sequencing services to its users using various Illumina

systems since 2008. During the last 14-years, its use increased by 26-fold and has provided services to 1000+ users from 30 institutions annually at 80% of the equipment’s capacity. For YCGA to continue to serve the need of its ever-increasing number of users, the current HiSeq 2500 must be replaced with a new mid

throughput sequencer, the AVITI system. The primary justifications for this request are the following: an urgent and compelling need to replace our existing medium-throughput Illumina HiSeq 2500 sequencing systems, which are more than seven years old and functionally obsolete (since these systems will not be supported by Illumina anymore), and a need to

reduce the turnaround time of data delivery for user prepared, R&D, and single-cell genomics samples. Moreover, the requested equipment is cost-effective and has sufficient throughput to support the projects cited in this application and beyond in supporting the needs of the growing number of investigators wanting to use

this cost-effective, cutting-edge technology. Twenty-nine investigators support our request for funding the AVITI system with apt justifications. They propose to use the AVITI system to study the roles of variants, identification, development of new genomics- based technologies, analysis of single expression and chromatin accessibility at a single-cell level, epigenetic

changes in psychiatric, cardiovascular, skin, infectious diseases, and cancer. The strengths of this proposal include: (1) An efficient and effective centralized facility serves an extraordinarily diverse and productive investigator user base. (2) The extensive experience of the PI. (3) The extensive

infrastructure and expertise are available to bring the requested instrumentation online and oversee its continuous use. Yale University has made a significant investment in capital and institutional talent to build a world-class genomic center that has proven highly successful. The requested instrumentation will leverage the

infrastructure to ensure its high value and broad impact on NIH-funded biomedical and basic research within and beyond the Yale community.