Microfluidic immunoassay as a polysubstance oral fluid screening tool for law enforcement to rapidly identify recent drug use in traffic stops to reduce traffic fatalities and injuries.

Abstract More Americans are driving under the influence of drugs (DUID) than ever before, leading to an increase in fatal motor

vehicle accidents. With more than 12 million drivers DUID each year, only a fraction of law enforcement officers (8%) has

the advanced training needed to recognize drug impairment in roadside traffic stops, representing about 220 drivers DUID

for every patrol officer with advanced training. Like the breathalyzer for alcohol, presumptive drug screening technology can aid patrol officers in making standardized and objective decisions for DUID arrest. However, current drug screening

tests are primarily based on immunochromatographic test strips, and, as a result, yield merely qualitative results with poor

diagnostic performance and offer limited drug test menus that are missing high-priority drugs like fentanyl. There is an

unmet need for drug screening technology with performance characteristics like laboratory tests and the ability to measure multiple high-priority drugs, including fentanyl. Toward this goal, Portable Diagnostic Systems is developing a next-

generation microfluidics-based drug screening tool called the Integrity-1 Analysis System. The Integrity-1 Analysis System detects low abundance targets in small volumes (