Human Immune Monitoring Core

ABSTRACT – Human Immune Monitoring Core The Human Immune Monitoring Core will support the Stanford HIPC projects with standardized, state-of-the- art, comprehensive immune monitoring services for the clinical samples collected across all three clinical studies in this application. The Core will leverage the considerable infrastructure of the Human Immune

Monitoring Center at Stanford, which has all the necessary space, equipment, expertise, and personnel to conduct these assays. Specifically, the Core will provide: (1) Olink immunoassays on serum, using their inflammation panel of 92 cytokines and related molecules; (2) CyTOF PBMC phenotyping with intracellular

cytokine staining (CyTOF ICS) and EpiTOF assays for DNA methylation in specific immune cell subsets; (3) BD Rhapsody single-cell transcriptomics assays with AbSeq and TCR targeting; and (4) Luminex assays for antibodies to SARS-CoV-2 proteins and antigens of other heterologous viruses. Data from these assays will be

organized, mapped to clinical demographics, and shared with the Data Management and Analysis Core, using our Stanford Data Miner online database. This will also facilitate the upload of these data sets to ImmPort. We anticipate that the data generated through the Core will be a resource to be mined well beyond the limits of the

HIPC grant.