MeDCAN - Methodology for Deuteration, Contrast variation and Analysis for Neutrons in Cancer

Virtually every process in life proceeds via membranes and their associated proteins.

In recent years neutron reflectometry (NR) emerged as a powerful technique to provide unique insight into membranes’ overall organization, thereby filling the gap between molecular features and cellular function.

However, current NR methods are mainly restricted to model membranes of reduced complexity which are lacking the functionality of real membranes in vivo.

Therefore, we will develop next-generation NR methodology for probing complex native-like biomembranes and associated protein assemblies.

By using the mitochondrial outer membrane (MOM) system and the Bcl-2 family of proteins involved in cell death regulation and cancer, we will generate a set of innovative tools and approaches for component specific deuteration, multidimensional neutron contrast optimization and data-analysis.

This approach will enable visualization of the individual lipid and protein components and their behaviour in-situ in realistic biomembranes of native-like complexity.

With this innovative next-level NR methodology on complex life-science systems will not only the users of the new ESS instruments benefit but also the current ISIS user community.

This front-line strategic method development for life sciences will also significant impact on providing the molecular mechanism by which Bcl-2 proteins exert their regulative apoptotic function at the MOM and are involved in many, often untreatable cancers.