Funktionell proteomik för precisionsmedicin

Cellular activity states are manifested by changes in protein concentration and distribution, but importantly, also by factors such as posttranslational modifications or dynamic interactions among the proteins. The latter is information that has been difficult to capture for patient samples.

Building on our long tradition of constructing DNA-assisted protein assays in solution and in situ, along with a series of recent developments, we will now establish dedicated panels for multiplex in situ detection of protein activity states to monitor responses to single or combinations of drugs. The assay panels will target critical sets of protein interactions and phosphorylations.

In the same assays we will also be able to characterize individual cells according to whether they carry specific mutations, or according to the target engagement of clinical drugs.

Armed with these panels we will collaborate with clinical colleagues to investigate drug responses by leukemic and solid tumor cells.

We plan to make the panels broadly available via SciLifeLab, and in a perspective beyond the present project these analyses can become an important means of therapy selection and follow-up in precision medicine.