Multiple correlative nanoscopy imaging of molecular turnover in nerve cells

Molecular turnover is a critical process in nerve cells reflecting dynamic intracellular changes related to cellular synthesis, metabolism, and degradation. Dysregulation of this turnover causes cellular dysfunction leading to cell death and neurodegeneration.

A critical need exists to develop novel approach (MultiCoN), integrating five state-of-the-art techniques with novel labelling probes to unify all the methods.

Overcoming the limitations of current technology, this will enable acquisition and integration of molecular information about nerve cells in a fashion previously not possible.

The project comprises the following aims: 1) develop integrated methods for correlative nanoscale bioimaging and analysis, 2) develop novel labelling probes for multimodal imaging, and 3) apply the MultiCoN concept to determine the molecular regulation of neural stem cell (NSC) differentiation, and neuronal activity.

MultiCoN offers the possibility to integrate multi-dimensional characteristics of the cells, opening new perspectives in our understanding of cellular function.

Application to NSCs will provide new insights into the molecular mechanism of NSC differentiation, and control over neural stem cell fate.