Potentiometric Nanoprobes for Continuous Real-Time Analysis at Single-Cell Level

This project addresses the growing demand for new analytical tools that target real-time and continuous single-cell analysis for ins and the proteome. The methodology is conceived to develop a disruptive sensing concept based on label-free potentiometric nanosensors.

Several innovation paths and research strategies will be explored for the development of versatile potentiometric nanoprobes, providing analytical figures of merit and performances that meet the requirements for cell measurements.

Investigations considering different cell lines before and after stimulation (pharmaceutical administration to the extracellular medium) will be performed.

The research approach, which is minimally perturbative for the cell and requires minimal costs, will promote a sustainable economic investment in single-cell analyses and will open new possibilities for fundamental research in related fields, including clinical investigations.

Accordingly, this project primarily targets sustainable development goals number 3 and 10 related to Good Health and Well-Being and Reduced Inequalities.

New insights in cell physiology and pathology are expected to lead to significant advances that will improve EU population health and quality of life, especially in relation to the EU Mission: Cancer. This project will develop cost-efficient analytical tools aligned to the United Nations 2030 Agenda.